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Rab1b knockdown alters YCV acidification.

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posted on 23.10.2015, 02:45 by Michael G. Connor, Amanda R. Pulsifer, Christopher T. Price, Yousef Abu Kwaik, Matthew B. Lawrenz

RAW264.7 macrophage cells were reverse transfected with scrambled (Scr), Rab1b or Copβ1 siRNA. 48 h after transfection cells were incubated with Lysotracker Red DND-99 for 1 h and then infected with live or paraformaldehyde-killed Y. pestis CO92 pCD1(-) pGEN222 expressing EGFP (MOI 7.5). Colocalization of Lysotracker Red DND-99 and Y. pestis CO92 pCD1(-) pGEN222 was determined by confocal microscopy. (A) Representative images showing colocalization of Lysotracker Red DND-99 and Y. pestis. Scale bar is 5μm. (B) Percent of YCVs that colocalized with Lysotracker Red DND-99 at 20 min post-infection. (C) Percent of YCVs that colocalized with Lysotracker Red DND-99 at 80 min post-infection. ** = p<0.01, *** = p<0.001.

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