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R. montanensis infection enhanced human cerebral microvascular endothelial cell permeability.

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posted on 20.02.2013, 04:00 by Bin Gong, Liang Ma, Yan Liu, Qinyu Gong, Thomas Shelite, Donald Bouyer, Paul J. Boor, Yong Sun Lee, Andres Oberhauser

Endothelial cells were seeded on type I rat-tail collagen-coated polycarbonate transwell filters and infected with R. montanensis at an MOI of 10 in triplicate, or mock infected. After 24, 48, and 72 hr, FITC-dextran was added to the upper chamber medium, and the presence of FITC dextran in the lower chamber was quantified after 3 hr. The results are expressed as the fold-increase in monolayer permeability over basal permeability levels (* p<0.05).