RT-qPCR and western-blot analysis of GFP expression.
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posted on 2013-06-20, 01:52 authored by Rafael Luis Kessler, Daniela Fiori Gradia, Rita de Cássia Pontello Rampazzo, Édio Elígio Lourenço, Nilson José Fidêncio, Lauro Manhaes, Christian Macagnan Probst, Andréa Rodrigues Ávila, Stenio Perdigão Fragoso(A) mRNA levels for the GFP and NEO genes in epimastigotes and metacyclic trypomastigotes, as determined by RT-qPCR and shown as relative mRNA levels, with the metacyclic level fixed at 1. (B) Western blot of GFP in epimastigotes (Epi) and metacyclic trypomastigotes (Meta). The “Loading control” (left) is the Ponceau-stained membrane before incubation with anti-GFP antibody (right). Note the detection of GFP only in epimastigotes.
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biotechnologyGenetic engineeringtransgenicsdrug discoverygeneticsMolecular geneticsGene regulationmicrobiologyProtozoologyParastic protozoansTrypanosomaMolecular cell biologycytometryflow cytometryDrugs and devicesDrug research and developmentInfectious diseasesParasitic diseasesProtozoan infectionswestern-blotgfp
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