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ROS production by P. acnes-stimulated keratinocytes.

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posted on 24.07.2009 by Philippe A. Grange, Christiane Chéreau, Joël Raingeaud, Carole Nicco, Bernard Weill, Nicolas Dupin, Frédéric Batteux

HaCaT cells were incubated for 18 h with P. acnes at an MOI of 50, 5, 0.5, and 0.05 (gray bars). Control experiments were done on HaCaT cells alone (black bars). Measurement of (A) superoxide anion, (B) nitric oxide, and (C) hydrogen peroxide was realized by spectrofluorometry as described in Materials and Methods. Data are means±SD of two separate experiments.