ROS production by P. acnes-stimulated keratinocytes.
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HaCaT cells were incubated for 18 h with P. acnes at an MOI of 50, 5, 0.5, and 0.05 (gray bars). Control experiments were done on HaCaT cells alone (black bars). Measurement of (A) superoxide anion, (B) nitric oxide, and (C) hydrogen peroxide was realized by spectrofluorometry as described in Materials and Methods. Data are means±SD of two separate experiments.