RIN3 interacts with endogenous BIN2.
figureposted on 2012-11-20, 00:07 authored by Christine Janson, Noriyuki Kasahara, George C. Prendergast, John Colicelli
(A) Cells were stimulated with 100 ng/ml SCF for indicated time points and lysates were immuno-precipitated with anti-RIN3 or beads alone (ctrl). Whole cell lysate (WCL) and IP samples were immunoblotted for RIN3 and BIN2. The immunoblots shown are representative of two independent experiments. At five minutes post-stimulation the amount of BIN2 precipitated was reduced by 81±6% compared to unstimulated (ImageJ), p<0.05. (B) Cells were stimulated with 100 ng/ml SCF for 0 or 5 minutes, then lysed immediately or resuspended in SCF free medium to recover for 4 hours at 37°C before lysis. Lysates were immunoprecipitated and immunoblotted as in A.