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RA induces Cyp26b1 expression in T cells.

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posted on 2011-01-07, 02:13 authored by Hajime Takeuchi, Aya Yokota, Yoshiharu Ohoka, Makoto Iwata

(A) Naïve CD4+ T cells were stimulated with antibodies to CD3 and CD28 in the presence or absence of AtRA (10 or 100 nM) for 2 days, and were further cultured with IL-2 and the same concentration without antibodies for 2 days. The cells were analyzed for the Cyp26 expression by RT-PCR. Rplp0 was used as a loading control. (B) Naïve CD4+ T cells were cultured as above but in the presence of graded concentrations of AtRA (0–100 nM). The mRNA levels were measured by quantitative real-time PCR. The expression levels are indicated as ‘fold induction’ relative to the level in the control cells without AtRA. (C) Naïve CD4+ T cells were cultured as above in the presence (squares) or absence (rhombuses) of 10 nM AtRA, and aliquots of the cells were harvested at 0, 6, 12, 24, 48, 72 and 96 h after the start of the culture. The mRNA levels were measured by quantitative real-time PCR. The expression levels are indicated as ‘fold induction’ relative to the level in the control cells without AtRA at 0 h. (B, C) Data are shown as the mean ± SD of triplicate cultures. Data are representative of three independent experiments.

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