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RANKL-independent osteoclast differentiation induced by TGFβ and TNF-α cytokines.

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posted on 21.02.2013, 12:24 by Yalei Wu, James Torchia, Wei Yao, Nancy E. Lane, Lewis L. Lanier, Mary C. Nakamura, Mary Beth Humphrey

BMMs from WT, DAP12-/- and DAP12-/-FcRγ-/- were separated and non-adherent preosteoclasts were cultured with RANKL (A, E, I); or pretreated by 2 ng/ml TGFβ (B, F, J) for 2 days, followed by 30 ng/ml TNF-α treatment (C, G, K) or 25 ng/ml RANKL (D, H, L) for another 2 day. TRACP staining was performed at the end of the experiment, as described. TRACP staining is indicated by dark purple. WT: wild-type mice; DKO: mice lacking both DAP12 and FcRγ. Experiment was repeated three times with representative results shown.