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Quinacrine increases the thermal stability of MglA and SspA.

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posted on 2013-01-23, 00:48 authored by Algevis P. Wrench, Christopher L. Gardner, Claudio F. Gonzalez, Graciela L. Lorca

Melting curves of purified (A) Ft-MglA (B) Ec-SspA and (C) Ft-MglA/Ft-SspA complex in absence or presence of increasing concentrations of quinacrine (125, 250 or 500 µM). Purified proteins (20 µM) were subjected to gradually increasing temperatures in the presence of the fluorophore SYPRO Orange. Fluorescence intensities were plotted against temperature and transition curves were fitted using the Boltzmann equation.