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Quantitative analysis of RanBPM in cytoplasmic and nuclear fractions and its association with chromatin.

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posted on 06.02.2015, 03:00 by Louisa M. Salemi, Sandra O. Loureiro, Caroline Schild-Poulter

A) Hela cell extracts were partitioned in cytoplasmic, nuclear soluble and chromatin fractions as described in Materials and Methods. Proportional amounts of each fraction were analyzed by western blot with the indicated antibodies. Left, representative western blot, with the percentage of each fraction loaded indicated above each lane. Right, bar graph representing the percentage of RanBPM protein present in each fraction. Data represent averages from three separate experiments with error bar representing SD. B) Hela RanBPM shRNA cells were transfected with pCMV-HA-WT-RanBPM and processed as in A.

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