Quantitative Real Time PCR validation of gene expression.
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RT-PCR validation was performed for nine genes (ICAM1, IFNγR1, IL1B, IL13Rα1, JAK2, AIF1, CCR1, ALAS2, TIMP2) in healthy control, lSSc-NoPAH, and lSSc-PAH samples. Bars indicate comparisons with statistically significant differential expression. Symbols indicate the level of significance between groups (asterisks p≤0.05, open circles p≤0.001).