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Quantitation of HSV-1 latency in CD8β−/− infected mice.

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posted on 02.04.2014, 03:22 authored by Kevin R. Mott, Sariah J. Allen, Mandana Zandian, Bindu Konda, Behrooz G. Sharifi, Clinton Jones, Steven L. Wechsler, Terrence Town, Homayon Ghiasi

Wild-type (WT) C57BL/6 and CD8β−/− mice were infected as described in Materials and Methods. Twenty-eight days PI, TGs from infected mice were harvested and quantitative PCR and RT-PCR were performed on each individual mouse TG. Each data point for gB DNA represents the mean ± SEM from 24 TGs. For LAT RNA, each data point represents 22 TGs for CD8β−/− and 18 TGs for WT mice from two separate experiments. Panels: A) gB DNA; and B) LAT RNA.

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