Purification and size exclusion chromatography of PaoD.
figure
posted on 2014-01-31, 04:09 authored by Ana Rita Otrelo-Cardoso, Viola Schwuchow, David Rodrigues, Eurico J. Cabrita, Silke Leimkühler, Maria João Romão, Teresa Santos-Silva(A) 12% SDS/PAGE of the purified protein after Ni-TED chromatography. (B) Size exclusion chromatography of PaoD. Freshly purified PaoD was analyzed by analytical size exclusion chromatography in 50 mM Tris-HCl, 300 mM NaCl, pH 8.0 using a Superdex 75 column. Inset: plot of the standard proteins. Size exclusion chromatography markers (Bio-Rad): gamma-globulin (158 kDa), ovalbumin (44 kDa), myoglobin (17 kDa), and vitamin B12 (1.3 kDa).
