Pulsed-field gel electrophoresis and Southern analysis of the three R. amblyommii AaR/SC plasmids.
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(A) 5 kbp ladder. (B, D and F) Replicate panels of AaR/SC. (C) Southern analysis of gel from panel B hybridized with digoxigenin-labeled DNA Invertase probe, specific for pRAM18. (E) Southern analysis of gel from panel D hybridized with digoxigenin-labeled hsp2 probe, specific for pRAM23. (G) Southern analysis of gel from panel F hybridized with digoxigenin-labeled recD probe, specific for pRAM32. Asterisks mark the putative linear monomer form of each plasmid and arrows indicate their predominant conformational isomers. Relative positions of ISE6 host cell mitochondrial, m, and chromosomal, c, DNAs are indicated to the right of the gel in panel B. Linear DNA marker positions in kbp are noted to the left of panel A.