Figure_4.tif (491.24 kB)

Proliferation of spleen cells after restimulation with antigen or peptide.

Download (0 kB)
figure
posted on 20.02.2013, 21:40 by Floris Fransen, Rachel M. Stenger, Martien C. M. Poelen, Harry H. van Dijken, Betsy Kuipers, Claire J. P. Boog, Jos P. M. van Putten, Cécile A. C. M. van Els, Peter van der Ley

Spleens were taken from the mice after immunizations and spleen cells were incubated for 4 days with medium, antigen, or peptides, after which [3H]thymidine incorporation was determined. Spleen cells of C57BL/6, TLR2−/−, and TRIF-deficient (TRIFdef) mice immunized with PBS (3 mice per group) or N. meningitidis P1.5-1,2-2 OMVs (6 mice per group) were restimulated with 1 µM of peptides 11–24/25 and 4–52/53 (A). Spleen cells of C3H/HeOuJ and C3H/HeJ mice immunized with PBS (3 mice per group) or N. meningitidis P1.5-1,2-2 OMVs (6 mice per group) were restimulated with peptide pool 6 (1 µM of each peptide, B). Spleen cells of C57BL/6, TLR2−/−, TRIF-deficient, C3H/HeOuJ, and C3H/HeJ mice immunized with PBS (3 mice per group) or whole cell pertussis vaccine (B. pertussis, 6 mice per group) were restimulated with 500 ng/ml of FHA antigen (C). Data are expressed as means of stimulation indices.

History

Licence

Exports