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Profile of RPhC of sle-IgGmix-dependent products of X-OP25 relatively deep hydrolysis (A) and analysis of the products of the hydrolysis corresponding to different peaks after RPhC by TLC (B) or massspectrometry (C–E): (–), relative fluorescence (A).

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posted on 09.03.2013, 12:15 authored by Anna M. Timofeeva, Pavel S. Dmitrenok, Ludmila P. Konenkova, Valentina N. Buneva, Georgy A. Nevinsky

Numbers of lines in panel B coincide with numbers of peaks on panel A; lanes C1, C2 and C3 correspond to the products of reaction mixture before their separation by RPhC, X-OP25 incubated in the absence of IgGs, and a free fluorescent compound X, respectively. The arrows (and numerals on panel B) indicate the positions of OPs of different length and compound X. Panel C demonstrates the MALDI spectrum of the products corresponding to peak 4, while panel D to peak 9 (Fig. 5A) and the intact X-OP25 before its hydrolysis, respectively. MALDI spectrum of all products of X-OP25 hydrolysis after 24 h of its incubation corresponding to non-fractionated reaction mixture is given on panel E. The length of the X-OPs is given on the bottom. See Materials and Methods for other details.