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Production of 11-cis retinol by Lamprey RPE65 in HEK293F cells.

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posted on 27.11.2012, 01:44 by Eugenia Poliakov, Alexander N. Gubin, Olivia Stearn, Yan Li, Maria Mercedes Campos, Susan Gentleman, Igor B. Rogozin, T. Michael Redmond

A: Normal-phase HPLC of retinol isomers from saponified retinyl esters isolated from HEK293F cells expressing Lamprey RPE65 and bovine LRAT (blue trace). B: Normal-phase HPLC of retinol isomers from saponified retinyl esters isolated from HEK293F cells expressing Lamprey RPE65 with Lamprey LRAT (red trace) or only Lamprey LRAT (green trace).

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