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Primer binding and extension by HIV-1 RT on unadducted and C10R- or C10S-adducted templates.

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posted on 19.09.2013 by Parvathi Chary, William A. Beard, Samuel H. Wilson, R. Stephen Lloyd

Primer extension assays using 11-mer primers on unadducted (73t) and adducted templates (73t*) were performed as described under MATERIALS and METHODS. U, S, and R represent unadducted, (+) and (-) anti-trans-BPDE adducted templates, respectively. The R- and S-adducted templates do not allow the formation of full-length products. The major site of termination after 11 incorporations is indicated.

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