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Primary inflorescence height of four independent transgenic lines overexpressing the At1g74450 open reading frame.

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posted on 2015-10-20, 03:12 authored by Anne M. Visscher, Eric J. Belfield, Daniela Vlad, Niloufer Irani, Ian Moore, Nicholas P. Harberd

(A) Graph showing the average primary inflorescence height in cm for four independent 35S:ORF lines compared to wildtype Col-0. Bars indicate standard error for each line (2: n = 31, 3: n = 27, 4: n = 30, 5: n = 20, Col-0: n = 117). The asterisks indicate statistically significant differences (p<0.01) between each 35S:ORF line and wildtype Col-0 based on one-way ANOVA followed by post hoc t-tests (two-tailed distribution, two-sample unequal variance) and Bonferroni correction. (B) Composite gel image showing semi-quantitative RT-PCR results for the four independent 35S:ORF lines and wildtype Col-0. The top half of the gel image shows RT-PCR products after 28 cycles generated by constitutive control Alpha-(α)-Tubulin primers [21]. The lower half of the gel image shows RT-PCR products after 28 cycles generated by gene-specific primers.

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