Primary hHSC express adrenoceptors and NPY receptors, upregulated in NAFLD cirrhosis.
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a, b) Semi-quantitative Rt-PCR and Western blot analyses showing that cultured activated hHSC express α1A, β1, β2 and β3 adrenoceptorsm but not absent α1B or α1D adrenoceptors as confirmed by protein level expression. PCR reactions were carried out for 35 cycles. c) Immunohistochemistry for hHSC, showed significant upregulation of α1A, β1, β2 and β3 in livers of patients with NASH cirrhosis patients compared to near normal controls. *p<0.05.