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Predictions of B-cell linear epitopes and intrinsically unstructured/disordered regions in rTc_11623.20 (A) and rTc_N_10421.310 (B).

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posted on 16.09.2014, 03:04 by João Luís Reis-Cunha, Tiago Antônio de Oliveira Mendes, Rodrigo de Almeida Lourdes, Daihana Rodrigues dos Santos Ribeiro, Ricardo Andrez Machado-de-Avila, Maykon de Oliveira Tavares, Denise Silveira Lemos, Antônia Cláudia Jácome Câmara, Carlos Chavez Olórtegui, Marta de Lana, Lúcia Maria da Cunha Galvão, Ricardo Toshio Fujiwara, Daniella Castanheira Bartholomeu

The complete sequences of the recombinant proteins were submitted to the BepiPred and IUPred algorithms. The dashed arrow corresponds to the complete amino acid sequence of the recombinant proteins. The orange boxes indicate linear B-cell epitopes as predicted by BepiPred, and the gray boxes indicate unfolded regions that were predicted by IUPred. The epitope region that contains the peptide screened in the immunoblotting for each one of the proteins is highlighted by a blue box.