Postnatal RGM and Neogenin expression in the habenula and fasciculus retroflexus.

In situ hybridization (A–C, G–I) and immunohistochemistry (D–F) on coronal mouse brain sections at P5 (A–F) and in the adult (G–I). Sections D–F are counterstained in blue with fluorescent Nissl. (A–C, G–I) At P5 and in the adult, in situ hybridization reveals strong expression of RGMa and RGMb in the medial habenula (MHb). The lateral habenula (LHb) strongly expresses RGMb, while only weak expression of Neogenin is detected in Hb. (D–F) Immunohistochemistry detects strong expression of RGMa and RGMb and weak expression of Neogenin in the fasciculus retroflexus (FR). 3V, third ventricle; CX, cortex; SM, stria medullaris; PVT, paraventricular thalamic nucleus. Scale bars A–I: 200 µm.