Population dynamics in the presence and absence of tryptophan.
A. Sorting region (white circle) of suppressed cells in the presence of tryptophan with 300 µM TMG. One hundred suppressed cells were sorted into each nutrient condition in the presence or absence of tryptophan and further cultured. B. Temporal changes in the cellular states in the presence (+Trp) and absence of tryptophan (−Trp) with 300 µM TMG. The insets indicate culture time after sorting. C. Growth curves of cell populations in the presence (+Trp) and absence (−Trp) of tryptophan with 300 µM TMG after cell sorting. The differences in greyscale represent replicates (n = 4 and 20 in the presence and absence of tryptophan, respectively).