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Physico-chemical characterization of HMW-bLf.

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posted on 2014-09-15, 03:00 authored by Fawzi Ebrahim, Jayanth Suryanarayanan Shankaranarayanan, Jagat R. Kanwar, Sneha Gurudevan, Uma Maheswari Krishnan, Rupinder K. Kanwar

A) Fourier transform infra-red (FTIR) spectra of HMW-bLf indicating characteristic peaks and compared with other forms of bLf. B) Differential scanning calorimetry thermograms of the different bLf forms C) SDS-PAGE showing the comparative resistance of HMW-bLf to Omnizyme (human digestive enzyme cocktail) treatment. It also gives an indication that HMW-bLf is an oligomer made of bLf monomers; because upon digestion HMW-bLf besides dimers and trimers does not release any other fragments lower than ∼78 kDa apart from the ones produced from the digestion of commercially pure NM-bLf.