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Phylogenomics of Lactobacillus rossiae DSM 15814T.

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posted on 2014-09-29, 03:10 authored by Maria De Angelis, Francesca Bottacini, Bruno Fosso, Philip Kelleher, Maria Calasso, Raffaella Di Cagno, Marco Ventura, Ernesto Picardi, Douwe van Sinderen, Marco Gobbetti

A) 16S neighbor-joining (NJ) tree, resulting from the alignment of the 16S rRNA-encoding gene of L. rossiae DSM 15814T and that of 20 selected representatives of the Lactobacillus genus (L. amylovorus GRL 1112, L. acidophilus 30SC, L. kefiranofaciens ZW3, L. crispatus ST1, L. helveticus DPC 4571, L. gasseri ATCC 33323, L. johnsonii NCC 533, L. delbrueckii subsp. bulgaricus ATCC 11842, L. sakei subsp. sakei 23K, L. rhamnosus ATCC 8530, L. paracasei subsp. paracasei, L. casei ATCC334, L. ruminis ATCC 27782, L. salivarius UCC118, L. fermentum IFO 3956, L. reuteri DSM 20016, L. plantarum WCFS1, L. brevis ATCC 367, L. buchneri NRRL B-30929 and L. sanfranciscensis TMW 1.1304). The corresponding 16S rRNA-specifying sequence of Bifidobacterium breve UCC2003 was used as an outgroup. The positioning of L. rossiae DSM 15814T within the Lactobacillus genus is indicated in red. B) Multilocus supertree resulting from the alignment of the sequence of three housekeeping genes (groEL, dnaK and grpE) of L. rossiae DSM 15814T and 20 selected representatives of the Lactobacillus genus (see above for the species). The positioning of L. rossiae DSM 15814T within the Lactobacillus genus is indicated in red. C) Multilocus supertree resulting from the alignment of 96 selected orthologous genes of L. rossiae DSM 15814T and 20 selected representatives of the Lactobacillus genus (see above for the species). The positioning of L. rossiae DSM 15814T within the Lactobacillus genus is indicated in red.

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