Phylogenetic relationships among vertebrate and invertebrate IκB members determined using the NJ method.
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(A) A total of 297 vertebrate protein sequences were included in this analysis (IκBα, IκBβ, IκBε, IκBζ, IκBNS, Bcl3, NF-κB1 and NF-κB2). (B) A total of 43 invertebrate protein sequences were included in this analysis (Cactus, Relish and NF-κB1). Bcl3 from Nematostella vectensis was considered as an outgroup. Bootstrap scores of >70% have been provided. Each IκB member is represented by a unique color in the phylogenetic tree: IκBα (magenta), IκBβ (red), IκBε (cyan), IκBNS (dark green), IκBζ (brown), Bcl3 (purple), Cactus (orange), Relish (black), NF-κB1 (blue), and NF-κB2 (light green). Taxa terminologies are presented as the IκB protein name followed by an abbreviated form of the species name. Clade organizations obtained for both vertebrate and invertebrate phylogenies are identical to all IκB phylogeny.