Phenotypic expression of resistance to cerebral malaria in mice from pedigree 48.
G3 and F2 mice homozygote (P48/P48) or heterozygote (P48/+) for the B6-derived mutant central chromosome 8 were identified by genotyping, and were subjected to several analyses, along with parental C57BL/6J, C57BL/10J and 129S1/SvlmJ controls. (A) Macroscopic examination of thymus from control and mutants showing severely atrophied thymus in homozygote mutants (representative of 5 mice per group). (B) FACS density plots of different cell populations in thymus (top), spleen (middle) and bone marrow (bottom) stained for CD4, CD8, CD19, and CD117. The position of the different cell lineages in the scatter plots are identified at the extreme right panel and their numbers are expressed as a percentage (± SE; n = 5 mice per group) of total cells in this tissue. (C) Flow cytometric analysis of immune cell lineage composition expressed as the absolute number (mean ± SD; n = 4–6 mice per group) of CD4+ and CD8+ single positive (SP), CD4+CD8+ double positive (DP), B cells (CD19+), granulocytes (GR; Gr1+), hematopoietic stem cells (HSC; lineage−CD117+) and NK cells from 105 cells from spleen, thymus and bone marrow. Asterisks (one-way Anova test with Bonferroni post-test) identify significant differences between experimental animals and the C57BL/6J controls: *p<0.05; ** p<0.01; *** p<0.001. Data are representative of two independent experiments.
