Phenotypic effect of dsRNA injection with methoprene and acetone treatments.

(A–D) Acetone-treated animals. (A) Size comparison of amp^r, vvl and Met dsRNA injected prepupae treated with acetone. (B, left) vvl dsRNA-injected prepupa. (B, middle) vvl dsRNA-injected prepupa with gin traps (arrow) developing underneath the cuticle. (B, right) vvl dsRNA-injected prepupa with complex eyes (arrowhead) developing underneath the cuticle. (C) amp^r dsRNA-injected adult. (D) Met dsRNA-injected eclosed adult. (E–H) Methoprene-treated prepupae. (E) Size comparison of amp^r, vvl and Met dsRNA-injected animals treated with methoprene. (F, left) vvl dsRNA-injected prepupa. (F, middle) vvl dsRNA-injected prepupa with gin traps (arrow) developing underneath the cuticle. (F, right) vvl dsRNA-injected prepupa with complex eyes (arrowhead) developing underneath the cuticle. (G) amp^r dsRNA-injected arrested pupa. (H) Met dsRNA-injected eclosed adult. Scale bars represent 0.5 mm.