Persistent pseudohypoxia results in a gradual decrease of Ambra1 and Beclin 1 protein levels.

HeLa cells were treated with 500 μM CoCl₂ and Ambra1 and Beclin 1 protein were detected at 0, 2, 6, 8, 10, 15, 18, and 24 hr after treatment. The graph shows the densitometric quantification of Ambra1 (A) and Beclin 1 (B) related to Actin. Values are mean ± SD of three independent experiments relative to control. *: P-value < 0.05 is considered statistically significant.