Peripheral edema in embryos at E14.5d.
(A) Edema is apparent in the E14.5 NFAT5−/− embryos (b) compared with a wild-type littermate (a). The arrow indicate the outer skin layer. (B) Histological comparison of NFAT5+/+ (a and c) and NFAT5−/− (b and d) embryonic heart at 14.5 days. (C) Representative photomicrograph showing proliferating cell nuclear antigen (PCNA)-positive cells (arrows) in compact zone and trabecular region in E14.5 NFAT5+/+ and NFAT5−/− hearts. (D) Representative photomicrography showing the (a and b) Bcl-2, (c and d) Bax and (e and f) cleaved caspase-3 staining (arrows) on E14.4 NFAT5+/+and NFAT5−/− hearts. Com, compact layer; E, endocardial cushion; Endo, endocardium; Ep, epicardium; LV, left ventricle; Tr, trabecular. n = 5 Scale bar = 50 µm.