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Peripheral edema in embryos at E14.5d.

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posted on 20.02.2013, 16:46 authored by Man Chi Mak, Ka Man Lam, Ping Kei Chan, Yu Bond Lau, Wai Ho Tang, Patrick Ka Kit Yeung, Ben Chi Bun Ko, Stephen Man Sum Chung, Sookja Kim Chung

(A) Edema is apparent in the E14.5 NFAT5−/− embryos (b) compared with a wild-type littermate (a). The arrow indicate the outer skin layer. (B) Histological comparison of NFAT5+/+ (a and c) and NFAT5−/− (b and d) embryonic heart at 14.5 days. (C) Representative photomicrograph showing proliferating cell nuclear antigen (PCNA)-positive cells (arrows) in compact zone and trabecular region in E14.5 NFAT5+/+ and NFAT5−/− hearts. (D) Representative photomicrography showing the (a and b) Bcl-2, (c and d) Bax and (e and f) cleaved caspase-3 staining (arrows) on E14.4 NFAT5+/+and NFAT5−/− hearts. Com, compact layer; E, endocardial cushion; Endo, endocardium; Ep, epicardium; LV, left ventricle; Tr, trabecular. n = 5 Scale bar = 50 µm.