Participation of nPKC and PKD1 in the feedback monomerization of the EGFR.

(A) Time traces of diffusion coefficient of EGFR^wt in the presence of the PKC activator PMA (upper) or under nominally Ca²⁺-free conditions (lower panel). In red, the time trace of cytosolic Ca²⁺ concentration monitored with Fura Red is shown. (B) Time traces of diffusion coefficient of eGFP-EGFR^wt in the cell coexpressing constitutively active mutant, mCherry-PKD1^S738/742E (upper), in the cell pre-treated with 10 μM CID755673, PKD1 inhibitor (middle), or in the cell coexpressing kinase deficient mutant, mCherry-PKD1^K612W (lower panel).