Palladin and a stimulatory trigger enhance HDF cell migration, invasion, and degradation of extracellular matrix.

A) Migration across a transwell was compared for HDF transfected with empty vector (EV), WT-palladin (WT), FX-palladin (FX) when exposed to normal complete media or wounding media. Data shown is representative average ± SEM of three independent experiments. (t-test, *, p-value<0.05) B) Invasion across a matrigel-covered transwell was compared for HDF cells treated as above in (A) when exposed to complete media, wounding media, or conditioned media from Panc-1 cells. Data shown is representative average ± SEM of three independent experiments. (t-test, **, p-value<0.01) C) HDF cells were transfected as above in (A) and plated onto coverslips coated with Texas Red-labeled gelatin. Representative images taken via IF are shown. DAPI (blue) was used to visualize nuclei. D) Protein lysates from HDF cells transfected as above in (A) were tested for RhoA activity. Each sample was run in triplicate in two independent experiments. Error bars indicate SD. (t-test, **, p-value<0.01) E) Proliferation assay of HDF cells transfected as above in (A). Data indicates mean ± SD for three independent wells.