PS2 protein is increased in microglia activated by IFNγ.
A) Western blot analysis demonstrating the PS2 C-terminus migrating at ∼20 kDa. Twenty-four hour stimulation with 10 u/ml IFNγ induces increased levels of PS2 expression in lysates prepared from primary microglia, while 100 ng/ml LPS does not induce PS2 expression. B) Quantitative densitometry on Western blots of lysates from 4 independent cultures shows significantly increased induction of PS2 by IFNγ. Data represent the mean ± SEM of 4 independent experiments (*p<0.05; Student's t-test). C) Twenty-four hour stimulation of primary microglia with IFNγ results in significantly increased PS2 mRNA. Data represent the mean ± SEM of 3 independent experiments (** p<0.01 by Student's t-test).