PPARγ∶ RXR overlap sites show higher validation rate than nonoverlapping sites.
ChIP Q-PCR was used for validation of binding sites. 4 populations of binding sites were chosen for validation: PPARγ monosites (no RXR sites within 200 bp), RXR monosites (no PPARγ sites within 200 bp), low confidence PPARγmoPET2∶RXR heterosites (within 200 bp) and high confidence PPARγ∶RXR heterosites (within 200 bp). High confidence PPARγ∶RXR heterosites shows, on average, higher ChIP-qPCR enrichment than PPARγ or RXR monosites and PPARγmoPET2∶RXR heterosites. Shown is the enrichment over an unspecific antibody control. Each data point represents mean value of 3 biological replicates, with error bars indicating the standard deviation.