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PMNs treated with MSU crystals release NETs.

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posted on 2011-12-16, 00:41 authored by Ioannis Mitroulis, Konstantinos Kambas, Akrivi Chrysanthopoulou, Panagiotis Skendros, Eirini Apostolidou, Ioannis Kourtzelis, Georgios I. Drosos, Dimitrios T. Boumpas, Konstantinos Ritis

PMNs from control subjects treated with MSU crystals for different time points. NETs are assessed by immunofluorescence microscopy after co-staining with DAPI (blue) and MPO (green). Control represents untreated PMNs incubated for 180 min. Scale bars, 30 µm. Original magnification 400x. One out of three independent experiments is shown. B) Percentage of NET releasing cells presented in panel A. Data are representative of three independent experiments and presented as mean ± SD. * P<0.05; ns = non significant compared to control cells.

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