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PMBPs suppress MMP-9 activity and expression.

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posted on 05.11.2015 by Jia Liu, Jing Bai, Guoqiang Jiang, Xinli Li, Jing Wang, Dachang Wu, Lawrence Owusu, Ershao Zhang, Weiling Li

The cells were treated with PMBPs (0, 5, 10, 25 μg/ml) for 24 h. (A) Activity of MMP-9 in the cell supernatant at various concentrations of PMBPs was examined by gelatin zymography assay. The white bands represent MMP-9 mediated gelatin digestion. (B) The protein expression of MMP-9 in A2780 cells at various concentrations of PMBPs was evaluated by Western blot. Histogram shows mean level of MMP-9 (±SD) from three independent experiments. MMP-9 protein level was expressed relative to loading control, β-Actin, and standardized to the non-treated control group. *P<0.05 and **P<0.01 compared to the control group.

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