PLC-IP3 signaling pathway is involved in Tg-induced myometrial contractions through the regulation of [Ca2+]i.
(A) Inhibitory effects of cumulative doses of 2-APB and NCDC on contractile responses after stimulation with Tg in rat myometrial strips (n = 3). Contractions were measured as the area under the curve (AUC) and expressed as a percentage of the response to 5 µM Tg. (B–E) Representative antibody reaction blots for the relative levels of MLC20 and pMLC20 in protein samples from Tg (400 nM) -treated or untreated rat myometrial cells in the presence or absence of 2-APB (2-APB inhibitor) (B) or U73122 (PLCβ inhibitor) (D). Signal intensities for MLC20 and pMLC20 from three different blots were used for the quantitative analyses (C, E). Data are expressed as means ± SEM. *** P<0.001 compared to control.