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PI103 sensitizes RD cells to DOX-induced apoptosis.

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posted on 31.12.2012 by Diana Marklein, Ulrike Graab, Ivonne Naumann, Tiandong Yan, Rosalie Ridzewski, Frauke Nitzki, Albert Rosenberger, Kai Dittmann, Jürgen Wienands, Leszek Wojnowski, Simone Fulda, Heidi Hahn

A) Levels of phospho-Akt, Akt, phospho-S6 ribosomal protein and S6 ribosomal protein were measured by Western blot analyses in RD, TP5014 and HT1080 cells after treatment with 1 µM or 3 µM PI103 as indicated. B) Upper panel: Proliferation was estimated by BrdU incorporation after treatment with 0.5 µM DOX and/or 3 µM PI103. Middle panel: Apoptosis was analyzed by FACS after treatment with 1 µM DOX and/or 3 µM PI103 or solvent. Data represent mean+SEM of at least three independent experiments performed in triplicates. Comparisons were made with ANOVA/Tukey’s testing. *P<0.05 compared to cells treated with solvent; #P<0.05 compared to cells treated with either drug alone. Lower panel: Protein levels of phospho-Akt, Akt and caspase 3 in cells treated with 1 µM DOX and/or 3 µM PI103 or respective solvents.

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