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PHA-4 is required for starvation survival.

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posted on 19.02.2010, 00:33 by Mei Zhong, Wei Niu, Zhi John Lu, Mihail Sarov, John I. Murray, Judith Janette, Debasish Raha, Karyn L. Sheaffer, Hugo Y. K. Lam, Elicia Preston, Cindie Slightham, LaDeana W. Hillier, Trisha Brock, Ashish Agarwal, Raymond Auerbach, Anthony A. Hyman, Mark Gerstein, Susan E. Mango, Stuart K. Kim, Robert H. Waterston, Valerie Reinke, Michael Snyder

(A) Loss of pha-4 leads to reduced starvation survival of first stage (L1) larvae. Wild-type (WT) worms were soaked in no RNAi, Cherry double-stranded RNA (dsRNA), or pha-4 dsRNA without food for the indicated times. To determine viability, triplicate samples were transferred to plates with food. Numbers of worms surviving past L1 were counted after 2 days. Results are an average of three independent experiments, n = 300–500 worms counted for each strain per experiment, error bars represent standard error. * = p<0.05. (B) Overexpression of pha-4 increases L1 starvation survival. PHA-4:GFP and outcrossed WT worms were subjected to starvation in liquid. Survival was determined as in (A). Results are an average of two independent experiments, n = 500–1900 worms counted for each strain per experiment, error bars represent standard error, p<0.0001 log rank (Mantel-Cox) test.

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