Fig_4.tif (1.37 MB)
Download file

PGE2-EP4 pathway is in charge of LF41-mediated attenuation of hepatic TNF-α expression.

Download (0 kB)
figure
posted on 2015-05-15, 03:50 authored by Pengfeng Jin, Yunbo Chen, Longxian Lv, Jianzhuan Yang, Haifeng Lu, Lanjuan Li

(A) ELISA for PGE2 secretion by the terminal ileum and total PGE2 levels in the liver of mice (n = 8) orally treated either for 10 days with PBS, L-LF41, or H-LF41, or for 3 weeks with PBS or H-LF41. * P < 0.05 PBS. (B) ELISA for hepatic IL-10 protein concentration of mice (n = 8) fed either PBS or H-LF41 for 10 days. P > 0.05 compared to PBS. (C) q-PCR for hepatic Cox1 or Cox2 mRNA levels of mice (n = 8) fed either PBS or H-LF41 for 10 days. Results are expressed as fold change relative to PBS. P > 0.05 compared to PBS. (D) Western blot assay for representative hepatic COX-1 and COX-2 protein levels of mice (n = 4) orally treated with either PBS or H-LF41 for 10 days. Hepatic COX-2 protein levels from a mouse receiving 2 h of stimulation with LPS (0.5 mg/kg BW; single IP injection) were determined as a positive control (left lane). (E) Mice (H-LF41-treated groups: n = 10 per group; PBS-treated groups: n = 8 per group) were pretreated with 10 days of PBS or H-LF41, either alone or combined with administration of either a specific inhibitor for PGE2 receptor EP-4, ONA-AE3-208 (I-EP4), or its vehicle (Vehicle). Hepatic Tnf mRNA levels were assayed by q-PCR 2 h after LPS treatment. Results are expressed as fold change relative to PBS+LPS.* P < 0.05; & P < 0.05 compared to H-LF41+LPS; n.s., non-statistical difference. All values except that of Western blot are shown as mean ± SEM. Results are representative of 2 similar experiments.

History