PDLPs, but not other PD proteins, are located at the EHM.
(A) PDLP1-GFP is located at PD (arrows) in both uninfected and infected tissue. During infection, PDLP1-GFP is also located at haustoria (right). (B) EHM association is specific to PDLPs as the PD markers PCBD1-mCit, TET3-YFP and MP17-GFP do not locate to the EHM. PDCB1-mCit and TET3-YFP locate to the developing encasement (n = 13/13 and n = 12/12 respectively) and MP17-GFP shows no association with haustoria (n = 5/5). Images are fluorescent data (left) and fluorescence/transmitted light overlays (right). (C) PDLP2-GFP and PDLP3-GFP are located at the haustoria periphery (n = 10/10 and n = 5/17 respectively). (D) and (E) Diagrammatic representation of the topology of the PDLP1 and synthetic mCit-TMCT proteins following cleavage of the signal peptide. PDLP1 has 2 extracellular DUF26 domains, the transmembrane (TM) domain and cytoplasmic tail (CT), which projects into the cell cytoplasm (cyt). In the mCit-TMCT variant, the TM and CT is fused to mCitrine. (F) mCit-TMCT localises to the EHM similar to PDLP1-GFP (n = 10/10). Asterisks, haustoria; yellow arrows, developing encasement; white arrows, PD. Scale bars are 10 µm.