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Overexpression of sim3 reversed the temperature sensitive growth in the asf1-33 mutant.

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posted on 2013-02-20, 09:31 authored by Katsuhiro Tanae, Tomitaka Horiuchi, Yuzy Matsuo, Satoshi Katayama, Makoto Kawamukai

(A) Cultures of L972 (asf1+), SKP605-33 (asf1-33-13myc-kanr), and SKP605-33 harboring pREP41 or pREP41-sim3 were serially diluted with sterilized water and spotted on YES plates. Plates were incubated at 26, 34 and 36°C. (B) Cell morphology of the cells used in (A) was observed microscopically.

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