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Osteochondral Scaffolds seeded with MSCs before (a, b) and after (c–f) subcutaneous implantation in nude mice.

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posted on 21.08.2008, 01:02 authored by Lorenzo Moroni, Doreen Hamann, Luca Paoluzzi, Jeroen Pieper, Joost R. de Wijn, Clemens A. van Blitterswijk

(a) Cell aggregates in the chondral compartment maintained a rounded morphology typical of chondrocytes; insert shows stable aggregate formation after 48–72 hours in chondrogenic media. (b) Cell attached and spread on the BCP particles in the bone part; insert shows methylene blue staining of attached cells on porous pillars. (c) Bone part of the osteochondral construct.: pores were filled with de novo bone (fuchsin red staining). Note the embedded osteocytes and the osteoblasts laying at the outer edge of the mineralized matrix. (d) Occasionally, hypertrophic cells with positive stained matrix could be seen in the pores (thionine). (e) Cartilage part of the osteochondral construct: Cartilage tissue could be observed in the chondral part. Cells exhibit a round, chondrocyte-like morphology, locate din lacunae and surrounded by positive extracellular matrix (thionine staining). (f) Hypertrophic cells in the center of mineralized matrix (fuchsin red staining) and embedded osteocytes could be also occasionally found. Scale bar: (a, b) 50 µm; (c–f) 200 µm; Insert in (a): 250 µm; (b) 600 µm.


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