Omp29 induces actin rearrangement and FAK phosphorylation in OBA9.

(A–C) The confluent OBA9 cells in the tissue culture flask were incubated for 1 hour in medium alone (A) or in the presence of Omp16 (10 µg/ml) (B) or Omp29 (10 µg/ml) (C). In order to stain F-actin, OBA9 cells removed from culture plate by trypsin-EDTA were permeabilized and stained with FITC-Phalloidin. (A) The solid histogram # shows the non-stained OBA9 and the open histogram in gray line * indicates the FITC-Phalloidin staining of OBA9. (B and C) The open histogram * indicates the FITC-Phalloidin stained OBA9 incubated with medium alone and the solid histograms display the staining of OBA9 stimulated with Omp16 (B) or Omp29 (C), respectively. (D and E) After stimulation of OBA9 cells with Omp16 (10 µg/ml) (D) or Omp29 (10 µg/ml) (E) for the time periods shown in the figure, whole cellular proteins dissolved in lysis buffer were immuno-precipitated with anti-total FAK antibody bound to GammaBind Plus Sepharose beads. The proteins pulled down with the beads were blotted onto NC membrane and reacted with anti-total FAK antibody or anti-phospho FAK antibody using Western-blot method.