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OVCA420 cells have defective mitochondria.

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posted on 23.05.2014, 03:24 by Usawadee Dier, Dong-Hui Shin, L. P. Madhubhani P. Hemachandra, Larissa M. Uusitalo, Nadine Hempel

A. Mitotracker red staining reveals that OVCA420 cells display defects in mitochondrial morphology compared to NOSE007, ES-2 and OVCA433 cells. Representative images show fragmented mitochondria (a&b, arrows) and the presence of large globular aggregates (c&d, arrows) in OVCA420 cells. B. Assessment of mitochondrial to nuclear DNA ratios by comparison of mtDNA 16S Ct/nDNA 18S Ct ratios in total DNA from ovarian cancer cell lines, using real time PCR analysis. C. OVCA420 cells display enhanced expression of the fission protein Drp1 D. Densitometric quantification of the full length and Drp1 splice variant (n = 3, ANOVA, Tukey's post test *p<0.05, ****p<0.0001 significant difference compared to NOSE007; #p<0.05, ####p<0.0001 significant difference compared to OVCA420). E. OVCA420 cells display a decrease in mitochondrial membrane potential as evident by the lack of red JC-1 aggregate accumulation and higher staining for JC-1 green monomers. F. Quantification of red/green JC-1 staining indicative of membrane potential (n = 35 cells, ****p<0.0001, t-test).