OM2 activated AMPK-PGC1a signaling pathway.

(a-b) the adipocytes were pre-incubated with compound C (20 μmol/l) for 1 h, then were treated or untreated for 24 h; (a) a representative western blotting result of the indicated proteins of p-AMPK and p-ACC, PGC1α and ATGL. AICAR (1 mmol/l) was used as a positive control; (b) Oxygen consumption and AMP: ATP ratio of the adipocytes with indicated treatmen. Upper is representative oxygen consumption curves; Middle is quantitative changes in the respiratory rate; Lower is ratio changes in AMP/ATP; (c-f) the adipocytes were transfected with PGC1α siRNA for 48 h, then were treated or untreated for 24 h; (c) a representative immunoblot of PGC1ɑ (upper) and quantitative analysis (lower); (d) a representative immunoblot of ATGL (upper) and quantitative analysis (lower); (e) mtDNA content; (f) mRNA levels of Pparα and Cpt1α. Values are mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01 versus control; #P < 0.05, ##P < 0.01 versus OM2 or OM treated group.