Number of DNA lesions per 10 kb in specific genes.

Cryopreservation induces different levels of DNA damage in zebrafish genes. DNA was extracted from fresh, incubated in cryoprotectants (CPAs), cryopreserved (Cryo) and H₂O₂ treated (3% for 30 min) (H₂O₂) zebrafish genital ridges. The formula employed for lesion rate calculation [33] estimates the number of lesions in different treated samples in comparison with fresh control samples (no lesions). Three independent experiments were done and three replicates were carried out in each of them. Error bars indicate standard error (SE). Results are represented as the mean ± SE. Statistical differences were analyzed by one-way ANOVA followed by SNK as a post hoc test. Asterisks represent significant differences (p< 0.05) between treatments for each gene.