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Nude mice injected with Ba/F3-BCR-JAK2 cells develop tumors.

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posted on 2012-02-27, 01:50 authored by Álvaro Cuesta-Domínguez, Mara Ortega, Cristina Ormazábal, Matilde Santos-Roncero, Marta Galán-Díez, Juan Luis Steegmann, Ángela Figuera, Eva Arranz, José Luis Vizmanos, Juan A. Bueren, Paula Río, Elena Fernández-Ruiz

Mice were subcutaneously injected with 107 Ba/F3-mock cells (left flank) and Ba/F3-BCR-JAK2 cells (right flank). (a) Left panel: white light photograph showing mice bearing a tumor only on the right side, where Ba/F3-BCR-JAK2 cells were injected (black arrows). Right panel: EGFP+ tumor (white arrows) captured on a digital photostation showing the reflected image of the mice (right side). No EGFP expression was detected when Ba/F3-mock cells were injected (left side). (b) Flow cytometry analysis of EGFP-expressing tumor cells. (c) RT-PCR analysis to detect BCR-JAK2 expression using BCR-B and JAK2-3 primers. RNA from three different tumors was analyzed compared with RNA from Ba/F3-mock and Ba/F3-BCR-JAK2 cells used as negative and positive controls, respectively. pLZR-BCR-JAK2: plasmid bearing BCR-JAK2 was used as positive control. MW, marker.

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