Nuclear envelope remnants are relatively fluid membranes despite their enrichment in cholesterol.

The top solid-state deuterium NMR spectrum corresponds to POPC-²H₃₁ small unilamellar vesicles (SUV) acquired at 10°C post equilibrium at 40°C for 22h. The second spectrum corresponds to POPC-²H₃₁ MLVs containing 30 mol% cholesterol acquired at 10°C after equilibration at 40°C. The bottom two spectra are of 0.1% nuclei incubated with SUVs of deuterated lipid (POPC-²H₃₁) for 30 min at 40°C. Labelled nuclear envelope remnants were equilibrated at 40°C for 22h and the ²H NMR spectrum was acquired post-equilibrium at 10°C for 4h (NER Tfin₁) and 20h (NER Tfin₂). The dashed lines show the plateau quadrupolar splitting enlargement of labelled nuclear envelope remnants post-equilibrium. Arrows show the plateau quadrupolar splittings used to calculate the order parameters. NMR spectra are representative of those obtained in a duplicate experiment.