Nuclear envelope remnants are relatively fluid membranes despite their enrichment in cholesterol.
The top solid-state deuterium NMR spectrum corresponds to POPC-2H31 small unilamellar vesicles (SUV) acquired at 10°C post equilibrium at 40°C for 22h. The second spectrum corresponds to POPC-2H31 MLVs containing 30 mol% cholesterol acquired at 10°C after equilibration at 40°C. The bottom two spectra are of 0.1% nuclei incubated with SUVs of deuterated lipid (POPC-2H31) for 30 min at 40°C. Labelled nuclear envelope remnants were equilibrated at 40°C for 22h and the 2H NMR spectrum was acquired post-equilibrium at 10°C for 4h (NER Tfin1) and 20h (NER Tfin2). The dashed lines show the plateau quadrupolar splitting enlargement of labelled nuclear envelope remnants post-equilibrium. Arrows show the plateau quadrupolar splittings used to calculate the order parameters. NMR spectra are representative of those obtained in a duplicate experiment.