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Nuclear envelope remnants are relatively fluid membranes despite their enrichment in cholesterol.

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posted on 2013-02-21, 07:06 authored by Marie Garnier-Lhomme, Richard D. Byrne, Tina M. C. Hobday, Stephen Gschmeissner, Rudiger Woscholski, Dominic L. Poccia, Erick J. Dufourc, Banafshé Larijani

The top solid-state deuterium NMR spectrum corresponds to POPC-2H31 small unilamellar vesicles (SUV) acquired at 10°C post equilibrium at 40°C for 22h. The second spectrum corresponds to POPC-2H31 MLVs containing 30 mol% cholesterol acquired at 10°C after equilibration at 40°C. The bottom two spectra are of 0.1% nuclei incubated with SUVs of deuterated lipid (POPC-2H31) for 30 min at 40°C. Labelled nuclear envelope remnants were equilibrated at 40°C for 22h and the 2H NMR spectrum was acquired post-equilibrium at 10°C for 4h (NER Tfin1) and 20h (NER Tfin2). The dashed lines show the plateau quadrupolar splitting enlargement of labelled nuclear envelope remnants post-equilibrium. Arrows show the plateau quadrupolar splittings used to calculate the order parameters. NMR spectra are representative of those obtained in a duplicate experiment.