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Northern blot analyses of Actin, ATPase, Hsp70 and CLIC mRNAs in B. cockerelli and D. citri.

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posted on 16.11.2011, 00:44 by Hada Wuriyanghan, Cristina Rosa, Bryce W. Falk

Total RNAs (1.5 µg) from B. cockerelli (BC) and D. citri (DC) were separated by denaturing agarose gel electrophoresis, transferred to nylon membranes and hybridized with 32P-UTP-labeled probes prepared using corresponding B. cockerelli sequences. 0.5–10 Kb RNA ladder (Invitrogen) was used on the same gel and the sizes are indicated. Labels A – D above each panel indicate the specific probe used for the corresponding blot. The exposure times for BC-Actin, BC-ATPase, BC-Hsp70 and BC-CLIC were 15 h, 2 h, 38 h and 38 h, respectively.