Normal Macrophage Chemotaxis to Initial Sites of ΔRD1 Infection

Overlay of DIC and fluorescent images showing the hindbrain ventricle (hv) of infected embryos. The hindbrain ventricle/brain (hv/b) boundary indicated by a white dashed line.

(A) ΔRD1-infected embryo 4 h postinfection with individual infected macrophages marked by arrowheads.

(B) ΔRD1-infected embryo 5 h postinfection in which individual infected macrophages (arrowheads) have migrated from the hindbrain ventricle and into the brain.

(C) WT-infected embryo 24 h postinfection with macrophages beginning to aggregate (white arrow) in the hindbrain ventricle. A second out-of-focus aggregate is to the left (yellow arrow). Scale bar, 100 μm.